Huntington's disease gene dispensable in adult mice
Woodruff Health Sciences Center | March 8, 2016
Aggregates of the huntingtin protein, visualized with electron microscopy. Image from HDBuzz.net via Creative Commons.
The results were published Monday, March 7 in Proceedings of the National Academy of Sciences.
Huntington's disease is caused by a gene encoding a toxic protein (mutant huntingtin) that causes brain cells to die. Symptoms commonly appear in mid-life and include uncontrolled movements, balance problems, mood swings and cognitive decline. A juvenile form of Huntington's disease also can appear during the teenage years.
Researchers led by Xiao-Jiang Li, MD, PhD and Shihua Li, MD, at Emory University School of Medicine, used genetically engineered mice in which the huntingtin gene can be deleted, triggered only when the mice are given the drug tamoxifen. Note: these mice do not produce toxic mutant huntingtin protein.
When the huntingtin gene is deleted at an age older than four months, these mice appeared to stay healthy, despite having lost their huntingtin genes in cells all over their bodies. They maintained their body weight and could complete tests of movement and grip strength as well as control mice.
In contrast with adults, engineered mice younger than four months old whose huntingtin gene was deleted developed lethal pancreatitis. The huntingtin gene encodes a large scaffold protein, with many interaction partners, which is thought to be involved in intracellular trafficking. The huntingtin gene is essential for embryonic development, and scientists have already shown that if mouse embryos don’t have it at conception, they die in utero.
“When it comes to gene suppression or editing strategies for Huntington’s disease, a major concern has been possible side effects because of huntingtin’s essential function,” says Xiao-Jiang Li, who is professor of human genetics at Emory University School of Medicine. “Our studies suggest that such concerns may be allayed if deletion of huntingtin occurs only in the adult brain or in older adults.”
However, it is possible that other more subtle alterations of behavior or memory are present in the huntingtin-deleted mice, he adds.
The strategy of lowering huntingtin expression is widely believed to be promising in treating Huntington’s disease, Li says. Additional research could make clear whether the post-natal role of huntingtin tapers off with age in humans in the same way that it does in mice.
Li also is affiliated with State Key Laboratory at Institute of Genetics and Developmental Biology, Chinese Academy of Sciences in Beijing.
The paper’s first author is Guohao Wang, now at the State Key Laboratory at Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.
The research was supported by the National Natural Science Foundation of China (91332206), the National Institute of Neurological Disorders and Stroke (NS041669, NS095279) and the National Institute on Aging (AG019206).